Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld STE Buffer (10X), 4 L
STE ReagentCommon reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, 10X solutionTechnical Information:1X concentration solution containsNaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
Research Products International Corp TG 10X Solution, 2 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol
BLD PHARMATECH CO LIMITED 4-(Hydroxymethyl)-4-methyltetrahydropyran, CAS Number: 502609-47-4, 1g, Purity: 97%
4-(Hydroxymethyl)-4-methyltetrahydropyran, CAS Number: 502609-47-4, 1g, Purity: 97%
Andwin Scientific TRIS 1M ST SOLUTION PH9 250ML
Tris 1.0 M Sterile Solution, pH 9.0, Ultra Pure Grade, 250ml
TARGETMOL CHEMICALS INC P62-mediated mitophagy inducer
Also available in 1 mL, 2 mg, 5 mg, 10 mg, 50 mg, 100 mg, 200 mg and bulk. Please contact Fisher for quotes. P62-mediated mitophagy inducer (PMI) is a regulator of mitophagy. Purity 99.35%
Bioworld Tris-Acetate-SDS Running Buffer (10X), 1 L
TRIS-Acetate-SDS Running Buffer (10X) All bioWORLD running buffers are prepared with high quality reagents. Technical Information:Dilute with deionized water to make 1X. 10X concentration1X contains: 50mM Tris base, 50mM Tricine, 3.5mM SDS pH 8.24
Bioworld TBS Buffer, Low Salt (10X), 4 L
Product DescriptionTris-buffered saline (TBS) is low salt used for Western blot washing with a concentration of 10X. It contains 500 mM Tris, 1.5M NaCl and has a pH level of 7.4.TBS is isotonic and non-toxic, and is used to dilute substances. This buffer is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
Boca Scientific Inc Coating Buffer, pH 7.4, Based on PBS, 500ml
Coating Buffer is designed for adsorptive immobilization of proteins and antibodies on plastic surfaces, such as microtiter plates, or other protein binding surfaces. Depending on the protein or the antibody, the pH of the Coating Buffer is crucial for efficient immobilization, as the pH-value can have an influence on the steric structure of proteins or antibodies. Coating Buffer is offered with a pH of 7.4, based on PBS, or 9.6, based on carbonate. For the optimization of the coating procedure of a newly developed immunoassay, we strongly recommend testing the two Coating Buffers side by side. Depending on the type of surface and the type of proteins or antibodies, the required incubation time can vary.
Teknova 1M Tris-HCl, pH 7.9. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Cell Signaling Technology Tris Buffered Saline (TBS-10X) 1 liter
Tris Buffered Saline (TBS-10X) 1 liter
Quality Biological Inc Tris (1 M) pH 9.8 1000mL
Suitable for molecular biology protein chemistry and biochemical applications.DNase RNase and Protease tested.Filtered through a 0.22 micron membraneDnase Rnase and Protease tested Manufactured in an ISO 90012015 certified facility.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.8 (0.2 micron filtered), 500 ml.
Bioworld TTTRIS-TRICINE)-SDS BUFFE
TT (TRIS-TRICINE)-SDS BUFFER, READY PACK 10X: Tris-Tricine-SDS Buffer Tris-Tricine SDS (TT-SDS) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more convent...