Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 M EDTAPH7.2
NC3588267 50 MM TRIS-HCL 10 M EDTAPH7.2
Bioworld Bis-Tris, 500 g
Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane A common biological bufferAn inert, zwitterionic buffer that exhibits only a very small shift in dissociation constantSpecifications:Ultra Pure GradepKa (25°C): 6.5+/-0.2Appearance: SolidBoiling Point : 466.18 °C at 760 mmHg (Predicted)Density : 1.35 g/cm3 (Predicted)Melting Point: 102-105 °CPH Value: 5.8-7.2
Biotang Inc Tricine-buffered Saline (5X) pH 8.0, 250 ml
Tricine-buffered Saline (5X) pH 8.0, 250 ml.
Medchemexpress LLC Nvp-TAE 684 | 761439-42-3 | MFCD11977634 | 99.7% | 614.20 | C30H40ClN7O3S | 1 ML
NVP-TAE 684 is a potent, selective small-molecule inhibitor of anaplastic lymphoma kinase (ALK) used in preclinical research to block the growth of ALCL-derived and ALK-dependent cell lines (reported IC50 2-10 nM). It is provided as both solid material and as a ready-to-use 10 mM solution in DMSO, and is characterized for purity suitable for in vitro studies.
- Potent ALK inhibition with low nanomolar IC50 values.
- Selective activity against ALK-dependent cell lines.
- Available as a 10 mM solution in DMSO for ready-to-use experiments.
- High purity (99.7%) suitable for research applications.
- Molecular weight 614.20 Da and formula C30H40ClN7O3S.
- Offered in multiple solid pack sizes for flexible dosing and storage.
- Documentation available including data sheet and SDS.
Biotang Inc Nylon Membrane stripping Buffer(10X), 250 ml
Nylon Membrane stripping Buffer(10X), 250 ml.
Chemscene ChemScene | 2,2-Difluorocyclopropan-1-amine hydrochloride | 250MG | CS-W000385 | 0.98 | 105614-25-3| MFCD07777150 | 129.54
ChemScene | 2,2-Difluorocyclopropan-1-amine hydrochloride | 250MG | CS-W000385 | 0.98 | 105614-25-3| MFCD07777150 | 129.54
Research Products International Corp Tris Glycine Buffer 10x Powdr
Dry homogeneous blend of molecular biology grade TRIS base and Glycine. Add 174.4g to make 1L of 10x Concentrate.
![eMolecules TERT-BUTYL 2-(HYDROXYMETHYL)-1-OXA-7-AZASPIRO[4.4]NONANE-7-CARBOXYLATE | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502198948.JPG-250.jpg)
eMolecules TERT-BUTYL 2-(HYDROXYMETHYL)-1-OXA-7-AZASPIRO[4.4]NONANE-7-CARBOXYLATE | 1g
AstaTech | TERT-BUTYL 2-(HYDROXYMETHYL)-1-OXA-7-AZASPIRO[4.4]NONANE-7-CARBOXYLATE | 1g | 624127283 | AT23569 | 95.000 | | 257.330 | C13H23NO4
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Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005313 TRIS HYDROCHLORIDE 100G
SAPPHIRE NORTH AMERICA ALUMINIUM CRYO TBS + CPS 15ML
Aluminium cryo tubes + caps, 15 ml, 29mmx 30mm / 100 pcs
Bioworld TTETRIS-TAPS-EDTA) BUFFER
This item has a minimum order quantity of 2 per supplier requirements. TTE (TRIS-TAPS-EDTA) BUFFER POWDER BLEND: - Proteomics Grade - An electrophoresis running buffer for DNA Sequencing that yields longer reads
Dojindo Molecular Technologies Inc EPPS ( 25G), Good's buffer, CAS: 16052-06-5, pka=8.00, Active pH 7.5-8.5, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.