Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000409966-logo.png-250.jpg){kind=logo}
Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G
TAPS is an aminosulfonate-based lysozyme stabilizer and connexin channel inhibitor. It helps maintain the native structure of lysozyme in aqueous solution at pH 7.0 and protects it from heat-induced denaturation. TAPS is also considered a critical compound for investigating the structure and function of connexin channels.
- Maintains native structure of lysozyme in aqueous solution at pH 7.0
- Protects lysozyme from heat-induced denaturation
- Directly inhibits heteromeric connexin 32/connexin 26 channels (Cx32/Cx26)
- Reduces connexin channel-mediated solute exchange in a recombinant liposome system
- Critical compound for investigating connexin channel structure and function
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659833.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G
HEPES, Free Acid is a commonly used biochemical reagent available in a 500g size for large-scale buffer preparation. This acid form of HEPES is highly effective in maintaining pH in the range of 6.8 to 8.2, making it ideal for biological applications where pH stability is crucial. It is commonly used in cell culture, protein analysis, and enzyme assays, ensuring that the pH remains constant over extended periods. HEPES is non-toxic to most cells, making it an excellent choice for maintaining physiological conditions during experiments. This bulk package is perfect for high-volume laboratories and applications that require frequent buffer preparation.
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 245 Grams of Powder, Makes 50 Liters of Solution
Murashige & Skoog (MS) Medium with MES Buffer and Vitamins is a high-quality powdered formulation used for plant tissue culture. This 245g powder makes 50 liters of a nutrient-rich solution, providing all essential macronutrients, micronutrients, and growth hormones. The addition of MES buffer ensures a stable pH environment for long-term culture, while the B5 vitamins promote cell growth and differentiation. This medium is ideal for in vitro propagation, genetic transformation, and plant regeneration studies. It is widely used in plant biotechnology labs for various applications including cloning, gene editing, and somatic embryogenesis.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hydrate | 1266615-59-1 | 99.9% | 50 G
MES hydrate is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is widely used in biochemistry and molecular biology experiments for applications such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Provides effective buffering capacity at approximately pH 6.15.
- Its pKa value changes with temperature, necessitating consideration for different experimental conditions.
- Exhibits good buffering properties in biological experiments.
- Shows poor affinity with metal ions, allowing its use in studies involving them.
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661338.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 500 G
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) in a 500G pack is a highly stable and versatile reagent used for preparing buffers in biochemical and laboratory applications. PIPES is an effective buffering agent that ensures a constant pH range ideal for a variety of biological and chemical processes. Its unique structure offers excellent pH stability, particularly in acidic conditions, making it suitable for sensitive biological experiments. PIPES is widely used in molecular biology, protein purification, and enzyme activity studies. It is especially beneficial for applications that require precise pH control over prolonged periods, such as electrophoresis and chromatography.
Crescent Chemical Co Inc MORPHOLINOPROPANE SULFONICACID
Morpholinopropane sulfonic acid analytical grade pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. Size - 100G Sto
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 100G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 100G
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 4.9 Grams of Powder, Makes 1 Liter of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (1 liter, 4.9 grams of powder) is specially formulated for plant tissue culture. The inclusion of MES buffer helps maintain a stable pH, which is essential for controlling the growth environment of plant cells. This medium is ideal for plant growth, regeneration, and shoot multiplication, providing the necessary vitamins and nutrients for cellular metabolism and tissue development. It is commonly used in plant breeding and research for producing healthy and viable plant cultures. The formulation ensures that plant cells receive the required nutrients to thrive, making it an important tool for plant biotechnology and commercial propagation.
Alkali Scientific MOPSO Sodium Salt, 1 Kg
MOPSO Sodium Salt, 1 Kg, is an essential buffering agent used to maintain pH stability in a variety of laboratory applications, especially in protein and nucleic acid studies. The MOPSO buffer provides reliable pH control in the range of 6.5 to 7.9, making it an ideal choice for enzyme assays, protein extractions, and other biochemical processes. The 1 kg size is perfect for large-scale experiments or labs that require frequent use of this buffer for ongoing research. MOPSO is also widely used in pharmaceutical and biotechnology labs, where consistent pH levels are crucial for reproducible results in complex biological assays.
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 24 Grams of Powder, Makes 5 Liters of Solution
Murashige & Skoog Medium with MES Buffer is designed for plant tissue culture and is available in a 5-liter size. This powder, containing 24 grams, makes 5 liters of solution, providing essential nutrients and pH stability with the inclusion of MES buffer. The buffer ensures the pH remains constant, which is vital for maintaining optimal growth conditions for plant cells. This medium is widely used in laboratories for plant cell culture, genetic studies, and regeneration research. MES buffer is particularly important in experiments that require precise pH control, making this medium an essential tool for researchers working with plant tissue cultures.
FUJIFILM BIOSCIENCES INC RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES w/oL-Glutamine is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas